Dengue IgG / IgM Ikizamini Cyihuta (Ikizamini cya Zahabu)

Virusi ya Dengue, umuryango wa serotype enye zitandukanye za virusi (Den 1,2,3,4), ni virusi imwe, ihishe, yuzuye-virusi ya RNA.Virusi zanduzwa n'umubu wo mu muryango wa Stegemyia uruma ku manywa, cyane cyane Aedes aegypti, na Aedes albopictus.Muri iki gihe, abantu barenga miliyari 2,5 batuye mu turere dushyuha muri Aziya, Afurika, Ositaraliya, na Amerika bafite ibyago byo kwandura indwara ya dengue.Bivugwa ko miliyoni 100 z’indwara ya dengue na 250.000 by’indwara ziterwa na dengue hemorhagie zangiza ubuzima buri mwaka ku isi hose-3.

Kumenyekanisha antibody ya IgM nuburyo bukunze kugaragara mugupima virusi ya dengue.Vuba aha, kumenya antigene zasohotse mugihe cyo kwigana virusi kumurwayi wanduye byagaragaje umusaruro ushimishije.Ifasha kwisuzumisha guhera kumunsi wambere nyuma yo gutangira umuriro kugeza kumunsi wa 9, icyiciro cyamavuriro yindwara kirangiye, bityo bigatuma hakiri kare kuvurwa hakiri kare4-.Ikizamini cya Dengue IgG / IgM Rapid cyateguwe kugirango hamenyekane antigen ya dengue ikwirakwizwa. muri serumu, plasma cyangwa amaraso yose.Ikizamini gishobora gukorwa nabakozi badahuguwe cyangwa bafite ubumenyi buke, badafite ibikoresho bya laboratoire.

Ikizamini cya Dengue IgG / IgM ni umuvuduko ukabije wa chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi ya conjugate yamabara ya burgundy irimo antengens ya dengue recombinant ibahasha ihujwe na zahabu ya colloid (dengue conjugates) hamwe ninkwavu IgG-zahabu, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (bande ya G na M) na umugenzuzi (C band).Itsinda rya G ryashizwemo mbere na antibody kugirango hamenyekane virusi ya IgG anti-dengue, itsinda rya M ryashyizweho na antibody kugirango hamenyekane virusi ya IgM anti-dengue, naho C itsinda ryabanje gushyirwaho ihene irwanya urukwavu IgG.

Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette yikizamini, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.IgG anti-dengue virusi niba ihari murugero izahuza na dengue conjugates.Ubudahangarwa bw'umubiri burafatwa na reagent yometse kuri bande ya G, igakora itsinda rya G ryamabara ya burgundy, byerekana virusi ya dengue IgG ibisubizo byiza kandi byerekana ko byanduye vuba cyangwa byongeye.Indwara ya IgM anti-dengue, niba ihari murugero, izahuza na conjugate ya dengue.Ubudahangarwa bw'umubiri burafatwa na reagent yabanje gutwikirwa ku itsinda rya M, igakora itsinda rya M ryamabara ya burgundy, ryerekana virusi ya dengue IgM ibisubizo byiza kandi byerekana ko yanduye.

Kubura ibigeragezo ibyo aribyo byose (G na M) byerekana ingaruka mbi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate utitaye ku ibara iterambere kuri buri tsinda rya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.