INCAMAKE N'ISOBANURO IKIZAMINI
Leptospirose iboneka ku isi yose kandi ni ikibazo cyoroheje kandi gikomeye ku buzima ku bantu no ku nyamaswa, cyane cyane mu turere dufite ikirere gishyushye kandi cyuzuye.Ibigega bisanzwe bya leptospirose ni imbeba kimwe n’inyamabere nini zororerwa mu rugo.Ubwandu bwa muntu buterwa na L. abajijwe, umunyamurwango wo mu bwoko bwa Leptospira.Indwara ikwirakwizwa n'inkari ziva mu nyamaswa zakira.
Nyuma yo kwandura, leptospire iba mu maraso kugeza isukuwe nyuma yiminsi 4 kugeza kuri 7 ikurikira umusaruro wa anti-L.ibaza antibodies, ubanza icyiciro cya IgM.Umuco wamaraso, inkari hamwe nubwonko bwa cerebrospinal nuburyo bwiza bwo kwemeza indwara mugihe cyicyumweru cya 1 kugeza 2 nyuma yo guhura.Gutahura serologiya ya antiL.antibodiyite zo kubaza nazo ni uburyo busanzwe bwo gusuzuma.Ibizamini birahari muriki cyiciro: 1) Ikizamini cya microscopique agglutination (MAT);2) ELISA;3) Ibizamini bya antibody itaziguye (IFATs).Nyamara, uburyo bwose twavuze haruguru busaba ibikoresho bihanitse hamwe nabatekinisiye batojwe neza.
Leptospira IgG / IgM ni ikizamini cyoroshye cya serologiya ikoresha antigene ziva muri L. ibazwa kandi ikamenya antibodiyite za IgG na IgM kuri mikorobe icyarimwe.Ikizamini gishobora gukorwa nabakozi badahuguwe cyangwa bafite ubumenyi buke, badafite ibikoresho bya laboratoire bigoye kandi ibisubizo biraboneka muminota 15.
IHame
Leptospira IgG / IgM Byihuta Ikizamini Cyigikoresho ni urujya n'uruza rwa chromatografi
immunoassay.Cassette yikizamini igizwe na: 1) padi ya conjugate yamabara ya burgundy irimo recombinant L. interrogans antigens yahujwe na zahabu ya colloid (Leptospira conjugates) hamwe ninkwavu IgG-zahabu conjugate, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (M na G band) na bande yo kugenzura (C band).Itsinda rya M ryashizwemo mbere na monoclonal anti-muntu IgM kugirango hamenyekane anti-L .. abaza IgM, G itsinda ryabanje gushyirwaho reagent kugirango hamenyekane anti-L.abaza IgG, na C band yabanje gushyirwaho ihene irwanya urukwavu IgG.
Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.IgM anti-L .. abaza niba bahari murugero bazahuza na Leptospira conjugates.Immunocomplex noneho ifatwa kuri membrane na antibody yabanje gushyirwaho anti-muntu IgM, ikora itsinda rya M ryamabara ya burgundy, byerekana L .. abaza IgM ibisubizo byiza byikizamini.IgG anti-L .. abaza niba bahari murugero bazahuza na Leptospira conjugates.Immunocomplex noneho ifatwa na reagent yabanje gutwikirwa kuri membrane, ikora ibara ryamabara ya burgundy, ryerekana L .. abaza IgG ibisubizo byiza byikizamini.
Kubura ibizamini byose (M na G) byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate hatitawe ku iterambere ryamabara kuri buri tsinda ryipimishije.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
