INCAMAKE N'ISOBANURO IKIZAMINI
Igituntu ni indwara idakira, yandura yatewe ahanini na M. TB hominis (bacillus ya Koch), rimwe na rimwe na M. TB bovis.Ibihaha nibyo byibandwaho, ariko urugingo urwo arirwo rwose rushobora kwandura.
Ibyago byo kwandura igituntu byagabanutse cyane mu kinyejana cya 20.Nyamara, kuba vuba aha hagaragaye imiti irwanya ibiyobyabwenge1, cyane cyane ku barwayi ba sida2, byongeye gushimangira igituntu.Indwara zanduye zagaragaye ko zigera kuri miliyoni 8 ku mwaka zipfa miliyoni 3 ku mwaka.Impfu zarenze 50% mu bihugu bimwe na bimwe bya Afurika bifite umubare munini wa virusi itera SIDA.
Gukekeranya kwa mbere kwa clinique hamwe nubushakashatsi bwa radiografiya, hamwe na laboratoire nyuma yo kwisuzumisha hamwe numuco nuburyo gakondo (s) mugupima TB5,6 ikora.Nyamara, ubu buryo bwaba butagira sensibilité cyangwa butwara igihe, cyane cyane ntibukwiye kubarwayi badashobora kubyara ibibyimba bihagije, smear-negative, cyangwa bakekwaho kuba bafite igituntu kidasanzwe.
Igituntu IgG / IgM Combo yihuta yateguwe kugirango igabanye izo nzitizi.Ikizamini cyerekana IgM na IgG anti-M.TB muri serumu, plasm, cyangwa amaraso yose muminota 15.Igisubizo cyiza cya IgM cyerekana ubwandu bushya bwa M.TB, mugihe igisubizo cyiza cya IgG cyerekana kwandura mbere cyangwa karande.Ukoresheje antigene yihariye ya M.TB, inagaragaza kandi IgM anti-M.TB ku barwayi bakingiwe na BCG.Byongeye, ikizamini kirashobora
bikorwa nabakozi badahuguwe cyangwa batoya badafite ibikoresho bya laboratoire bitoroshye.
IHame
Igituntu IgG / IgM Byihuse Ikizamini ni chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi yamabara ya burugundy irimo antigene ya M.TB ihujwe na zahabu ya colloid (M.TB conjugates) hamwe ninkwavu IgG-zahabu conjugate, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (bande M na G. ) hamwe nitsinda rishinzwe kugenzura (C band).Itsinda rya M ryashizwe mbere na IgM ya monoclonal anti-muntu IgM kugirango hamenyekane IgM anti-M.TB, itsinda rya G ryabanje gushyirwaho reagent kugirango hamenyekane IgG anti-M.TB, kandi C itsinda ni pre -yambitswe ihene irwanya urukwavu IgG.
Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.IgM anti-M.TB niba ihari murugero izahuza na conjugate ya M.TB.Immunocomplex noneho ifatwa kuri membrane na antibody yabanje gushyirwaho anti-muntu IgM, ikora itsinda rya M ryamabara ya burgundy, byerekana ibisubizo byiza bya M.TB IgM.IgG anti- M.TB, niba ihari murugero, izahuza na M.TB conjugates.Ubudahangarwa bw'umubiri burafatwa na reagent yabanje gutwikirwa kuri membrane, bigakora ibara rya burgundy rifite amabara, byerekana ibisubizo byiza bya M.TB IgG.Kubura ibizamini byose (M na G) byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate hatitawe ku iterambere ryamabara kuri buri tsinda rya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
