TP Antibody Yipimisha Urupapuro

Syphilis Tp ni bacterium ya spirochete, ikaba ari yo itera sifile ya virusi.Nubwo umubare wa sifilis wanduye muri Amerika ugenda ugabanuka nyuma y’icyorezo cya sifilis, umubare w’indwara ya sifilis mu Burayi wazamutse kuva mu 1986 kugeza mu 1991. Mu 1992, abantu 263 bari hejuru cyane cyane muri Federasiyo y’Uburusiya.Ishami ry'Umuryango w'Abibumbye ryita ku Buzima ryatangaje ko abantu miliyoni 12 banduye mu 1995. Kugeza ubu, igipimo cyiza cy’ibizamini bya sifile sifile ku banduye virusi itera sida cyiyongereye vuba aha.
Kumenyekanisha byihuse antibody ya sifilis ni uruhande rwa chromatografiya immunoassay.
Ibikoresho byo kwipimisha birimo: 1) recombinant Tp antigen IgG zahabu ya conjugate ihuza ibara ryumutuku wa conjugate pad colloidal zahabu (Tp conjugate) ninkwavu.
2) Nitrocellulose membrane strip band irimo ibizamini (T) hamwe na bande yo kugenzura (C band).T band yabanje gushyirwaho antigen ya conjugate recombinant Tp antigen, naho C band yabanje gusiga ihene irwanya urukwavu IgG antibody.
Iyo ingano ihagije yicyitegererezo igabanijwe mu mwobo w'icyitegererezo, icyitegererezo cyimukira kuri karito nigikorwa cya capillary muri karito.Niba antibody ya anti Tp ihari murugero, izahuza na Tp conjugate.Uru rugingo rukingira umubiri noneho rufatwa kuri membrane na Tp antigen yabanje gutwikirwa, ikora umurongo wumutuku wumutuku T, byerekana ibisubizo byiza bya antibody ya Tp.Kubura kwa T band byerekana ko ibisubizo ari bibi.Ikizamini kirimo kugenzura imbere (band C) kigomba kwerekana ihene yumutuku wihene irwanya urukwavu IgG / urukwavu IgG zahabu conjugate yumubiri, utitaye kuri T-band.Bitabaye ibyo, ibisubizo byikizamini bitemewe kandi ikindi gikoresho kigomba gukoreshwa.